Test Kits for Marine Toxins

Marine Toxins Test Kits are kits to aid in the qualitative or quantitative testing of marine toxicants. These chemical compounds that cause harm are produced by micro-sized algae that reside in oceans and, occasionally, in lakes. They bioaccumulate in shellfish and other seafood and could cause illness when consumed.

<img width="458" src="https://5.imimg.com/data5/XO/GR/MY-1095393/milk-adulteration-test-kit-500x500.jpg">

Marine toxins have a severe impact on the fishing industry. They can lead to a negative perception among the public about shellfish, health problems caused by it and directly affecting economic loss. These toxins also account for millions of food-borne diseases every year. Marine toxins can be detected through MS/LC-MS. However these tests are costly and labor-intensive and require a high level of technical education. Consequently, many laboratories still use rapid test kits (ELISAs and LFAs) for the determination of these toxic substances. The effectiveness of these kits is not systematically assessed.

The poisoning of shellfish is caused by Diarrhetic Shellfish Toxins. They are produced in Dinophysis dinoflagellates. The detection of DSTs is crucial to protect the global seafood supply, safeguard public health and support the development of the industry. Current rapid methods of their detection are not reliable. In this study, we evaluated the results of LCMS/MS, LC-MS/LC and LCMS/LC in four labs with the results of five tests that are commercially available (quantitative Enzyme-Linked Immunosorbent Assays (ELISAs) and lateral flow tests) on spiked and naturally contaminated shellfish samples - Sydney Rock Oysters (Saccostrea glomerata), Pacific Oysters (Magallana gigas/Crassostrea gigas), Blue Mussels (Mytilus galloprovincialis) and Pipis (Plebidonax deltoides/Donax Deltoides).

Toxins were evaluated by contract laboratories using a combination DST analogues: OA 35 uL/3 g (10 LOR x 0.1mg/kg) as well as the DTX-1 7.5 uL/3 g and DTX-2 5.5 uL/3 of a gram. Toxin recovery was determined and compared with the spiked concentrations and toxin levels of certified reference material CRM (OA/DTX-1/DTX-2) as well as across laboratories.

The four ELISAs provided false positive results if the spiked OA was within or above the limit of regulatory. Furthermore, the Abraxis PP2A ELISA and the EuroProxima ELISA gave a large amount of results out of the range or limit when tested using blank matrixes.

https://mzfoodtest.com/ performed better than the ELISAs. However they all showed that the lateral flow assays had low reactivity to DSP congeners, which are found in high levels in naturally contaminated shellfish samples, particularly DTX-2 and GTX-1 (due to their need to undergo hydrolysis to release ester form). The Abraxis PP2A ELISA was discovered to have a particularly low response to the DSP congener DTX-3.

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Pub: 07 Jul 2023 17:24 UTC
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