Biodiversity monitoring at the community scale is a vital element of assessing and learning species distributions, ecology, variety, BloodVitals SPO2 and movements, and it is vital to understanding and monitoring environmental and anthropogenic effects on natural ecosystems.1, 2, 3, four Vertebrates in terrestrial ecosystems are experiencing extinctions and declines in each population numbers and sizes due to rising threats from human actions and environmental change.5, 6, 7, 8 Terrestrial vertebrate monitoring utilizing current methods is generally expensive and laborious, and though environmental DNA (eDNA) is turning into the software of alternative to evaluate biodiversity, few sample types successfully seize terrestrial vertebrate range. We hypothesized that eDNA captured from air may allow easy collection and characterization of terrestrial vertebrate communities. We filtered air at three localities within the Copenhagen Zoo: a stable, outside between the out of doors enclosures, and within the Rainforest House. Through metabarcoding of airborne eDNA, we detected 49 vertebrate species spanning 26 orders and 37 families: 30 mammal, 13 hen, four fish, 1 amphibian, and 1 reptile species.

These spanned animals saved at the zoo, species occurring in the zoo surroundings, BloodVitals SPO2 and species used as feed within the zoo. The detected species comprise a range of taxonomic orders and families, sizes, behaviors, and abundances. We discovered shorter distance to the air sampling device and better animal biomass to increase the probability of detection. We hereby present that airborne eDNA can offer a fundamentally new manner of studying and monitoring terrestrial communities. Lynggaard et al. display that airborne environmental DNA coupled with metabarcoding and excessive-throughput sequencing can be used to detect terrestrial vertebrates. The 49 detected species are identified to occur in or around the zoo research site. Animals in nearer proximity to the sampler and current in bigger biomass have greater detection probability. The air is filled with particles, equivalent to fungal spores, bacteria, vira, pollen, dust, sand, droplets, and fibrous materials, which may be airborne for BloodVitals experience days and transported over long distances.9,10 These contain DNA and/or carry DNA connected to them, and DNA sequencing has been used to determine the taxonomic origins of airborne fungal spores, algae, pollen, and microbiota collected on adhesive tape, in air filters, and in dust traps.11, 12, 13, 14, 15 Further, BloodVitals experience two current research demonstrated vertebrate detection via DNA filtered from air in small, confined rooms containing tens to a whole bunch of people of the target species,16,17 and one examine sequenced DNA from atmospheric dust samples in the global Dust Belt over the Red Sea and detected eukaryotes, together with small portions of human, cetacean, and chicken DNA.18 However, the use of airborne environmental DNA (eDNA) for learning and monitoring native vertebrate communities in a wider context has been unexplored.

We detected vertebrate airborne eDNA in Copenhagen Zoo, Denmark, by filtering air with three air sampling gadgets.

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Pub: 27 Nov 2025 02:48 UTC

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