This is based on the kinetics of nocodazole-induced Golgi fragmentation, https://pooct.nimsite.uk/assets/video/pnb/video-free-slots-no-deposit-win-real-money.html which is more much like the kinetics of accumulation of Golgi residents within the ER upon therapy with dominant detrimental Sar1 than to the kinetics of Golgi-to-ER transport induced by BFA (Storrie et al., 1998). In contrast to this proposal, our observation that the nocodazole-induced Golgi-to-ER transport pathway was H89 delicate (Figure 3, C and D) advised that the nocodazole-induced retrograde pathway was mechanistically more associated to both BFA- and https://recomendador-ia.barlovento.estudioalfa.com/assets/video/pnb/video-play-free-online-slots.html hypotonically induced retrograde transport than to the constitutive Golgi-to-ER transport pathway.

H89 remedy results in displacement of Sec13 from peripheral ER exit websites to a soluble cytosolic pool. These outcomes steered that H89 induced the redistribution of Sec13 from ER exit sites to the cytoplasm. As expected, Sec13 antibodies in untreated cells stained punctate, https://pooct.nimsite.uk/assets/video/pnb/video-panda-slots.html peripheral buildings (Figure 7A) presumably corresponding to ER exit sites (Tang et al., 1997). Strikingly, 50 μM H89 led to a fast reduction in Sec13 staining in peripheral ER exit websites over time such that by 10 min of H89 treatment, [http://f.r.A.G.Ra.nc.E.rnmn%[email protected]](http://f.r.A.G.Ra.nc.E.rnmn%[email protected]/?a[]=%3Ca%20href=https://pooct.nimsite.uk/assets/video/pnb/video-free-slots-no-deposit-win-real-money.html%3Ehttps://pooct.nimsite.uk/assets/video/pnb/video-free-slots-no-deposit-win-real-money.html%3C/a%3E%3Cmeta%20http-equiv=refresh%20content=0

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Pub: 29 Jan 2026 17:36 UTC

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